英语翻译To test our hypothesis that the gene block between duplicatedrrnS failed to amplify in Yu et al.'s study,we synthesizedthe three primer pairs used by Yu et al.(afterremoving mismatches based on our C.hongkongensissequences to improve spec

英语翻译To test our hypothesis that the gene block between duplicatedrrnS failed to amplify in Yu et al.'s study,we synthesizedthe three primer pairs used by Yu et al.(afterremoving mismatches based on our C.hongkongensissequences to improve spec
英语翻译
To test our hypothesis that the gene block between duplicated
rrnS failed to amplify in Yu et al.'s study,we synthesized
the three primer pairs used by Yu et al.(after
removing mismatches based on our C.hongkongensis
sequences to improve specificity).As expected,the three
shorter products mentioned in Yu et al.' paper were successfully
obtained (Table 4,Figure 2).We increased the
elongation time for PCR trying to obtain the longer fragments,
but failed probably because of distance between
the duplicated genes (2,147 bp) is too long.We designed
two new pairs of primers targeting the block between the
duplicated rrnS genes,with one primer of each pair
located in the rrnL gene that was supposed to be absent
according to Yu et al.(Table 4,Fig,1).The two new primer
pairs designed by us successfully amplified and produced
fragments of expected sizes,2,658 and 1,905 bp (Table 4,
Figure 2),proving that the gene block between the duplicated
rrnS genes are actually there.To further confirm that
the two products both contain the duplicated rrnS,each
product was used as PCR template for amplification with
the primers 2* that amplifies rrnS only; both PCR produced
a fragment of the expected size (824 bp),the same
as using genomic DNA as template (Figure 2).We also
sequenced some of the fragments,and the sequences are
the same as expected from the mt sequences we obtained.
These results clearly demonstrate that the duplicated rrnS
and the split rrnL exist in the mt genome of C.hongkongensis.
There is no loss of the duplicated genes and the gene
block between them."Tandem duplication-random loss"
is not a real feature of oyster mt genomes and has not
occurred during the evolution of C.hongkongensis.The
possibility of Yu et al.sequenced a rare mutant of C.hongkongensis
is extremely low considering:1) we sequenced
three individuals from three diverse populations; 2) Yu
and colleagues screened more than one individual; and 3)
we duplicated their results with our samples.This is a clear
case of PCR artifacts involving duplicated genes.

英语翻译To test our hypothesis that the gene block between duplicatedrrnS failed to amplify in Yu et al.'s study,we synthesizedthe three primer pairs used by Yu et al.(afterremoving mismatches based on our C.hongkongensissequences to improve spec
要测试我们对Yu等人研究中的未能放大位于复制rrnS中的基因块的假设,我们综合了Yu中所用的3对引子对（核酸引子?）（在根据我们C.hongkongensis序列移除不匹配结果来改善特异性之后）.正如预期的,成功获取了在Yu等人的著作中提到的三对较短的产物（表4,图2）.我们为PCR增加了延展时间,试图获取更长的片段,但是失败了.这可能是因为复制基因（2,147bp）间的距离太长.我们针对复制rrnS基因间的基因块设计了2对新的引子,其中每对中的一个引子都位于rrnL基因,这在Yu等人的研究（表4,图1）中本应是没有的.我们设计的2对新的引子成功地放大并生成了预期大小的片段,2,658和1905bp（表4,图2）,证明了在复制rrnS基因间的基因块确实存在.为了更多地确认这2个产物都包含有复制rrnS,每个产物都用作PCR模板用于放大,第二个引子只用来放大rrnS；两个PCR都生成了一个预期大小（824bp）的片段,跟使用染色体组DNA作为模板的一样（图2）.我们还测序了一些片段,而且序列跟我们得到的mt序列一样.这些结果清晰地表明了,复制rrnS和分裂rrnL是存在于C.hongkongensis的mt染色体组中的.它们之间的复制基因和基因块都没有损失.串联重复随即损失不是牡蛎mt基因组的一个特征,而且在C.hongkongensis的进化期间也没有出现.Yu等人极低地考虑了测序C.hongkongensis的一种罕见突变的可能性：1）我们从三个不同种群中测序了三个个体；2）Yu和他的同事们筛选出了不止一个个体；3）我们用我们的样例重现了他们的结果.这是一个涉及复制基因的PCR人工产物的清晰案例.
好高深的生物学啊.要不是出现个牡蛎还不知道是研究什么生物呢~语文不太好.翻译的比较生硬.

祝你找到合适的翻译，我看了看好像是关于什么产品方面的沟通，没想到这么多年不看英语，什么都不懂了。。