一个分子细胞生物学的问题Liver cancer is a top concern in China,Faculty at School developd a new drug candidate LIV that inhibit the proliferation of a liver cancer cell line HepG2.however the gene target is unknown.you are hired as a grou

一个分子细胞生物学的问题Liver cancer is a top concern in China,Faculty at School developd a new drug candidate LIV that inhibit the proliferation of a liver cancer cell line HepG2.however the gene target is unknown.you are hired as a grou
一个分子细胞生物学的问题
Liver cancer is a top concern in China,Faculty at School developd a new drug candidate LIV that inhibit the proliferation of a liver cancer cell line HepG2.however the gene target is unknown.you are hired as a group leader in the LIV target gene identification project.the mile stone of this project are:1.Establish the project of LIV treatment cased gene expression changes(10).2Verification of the gene expression of top 5gene that have the most change after LIV treatment using other methods(10).3.Predict the gene function(10).4.identify promoter region that is important for the LIV effect(10).5,purify the protein that binds to the promoter region identified 4(10)
可以带电脑的开卷考试，正在考。
大概说下也行

一个分子细胞生物学的问题Liver cancer is a top concern in China,Faculty at School developd a new drug candidate LIV that inhibit the proliferation of a liver cancer cell line HepG2.however the gene target is unknown.you are hired as a grou
(1) the best way is to set a gene chip test to compare the whole genome wide expression changes of proteins on different LIV drug concentration to the control. And then pick out the genes that changes significantly to control.
(2) we can use real-time PCR to test the top 5 gene expression levels on the treatment of LIV.
(3)Utilizing of system biology or bioinformatic methods to product the possible gene function of interest.
(4) base the genes of identification by gene-chip and real-time PCR methods, we can select a region of about 2000 bp upstream from start coding, and test the exact elements compositions of promoter.
(5) using CHiP technology we can screen out the proteins that binds to the promoter region identified