DNA酶反应实验的dna琼脂的配制是按照什么标准配制的?
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DNA酶反应实验的dna琼脂的配制是按照什么标准配制的?
DNA酶反应实验的dna琼脂的配制是按照什么标准配制的?
DNA酶反应实验的dna琼脂的配制是按照什么标准配制的?
Most agarose gels are made between 0.7% and 2%.A 0.7% gel will show good separation (resolution) of large DNA fragments (5–10kb) and a 2% gel will show good resolution for small fragments (0.2–1kb).Some people go as high as 3% for separating very tiny fragments but a vertical polyacrylamide gel is more appropriate in this case.Low percentage gels are very weak and may break when you try to lift them.High percentage gels are often brittle and do not set evenly.
You may take a look at the table:
Recommended % Agarose Optimum Resolution for Linear DNA
0.5 1,000–30,000bp
0.7 800–12,000bp
1.0 500–10,000bp
1.2 400–7,000bp
1.5 200–3,000bp
2.0 50–2,000bp